dotBLOT and slotBLOT

dotBLOT and slotBLOT dot and slot blotters RNA BIO-DOT Adapted from existing protocols by Vinh Pham Last modified: July 8, 2003 MATERIALS: DRY Bio-Rad Bio-Dot SF Microfiltration dot slot auction Dot blot is a technique for detecting, analyzing, and identifying proteins This technique is similar to western blot, but protein samples

dot slot auction One such immunodetection method is widely known as Dot Blot The method involves applying small volumes of antigens or test samples on protein binding membrane;

dot and slot blotters The units incorporate precision lapped surfaces to ensure uniform blotting membrane contact and a leak proof gasket These prevent lateral transfer of samples Dot Blot · Membrane Preparation · Block · Wash · Primary Antibody Incubation · Wash · Secondary Antibody Incubation · Wash · Detection